[Objective] The aim was to find out the optimum conditions for separation and purification of total saponins from RADIX ASTRAGALI.[Method] Employing ultraviolet spectrophotometry as dynamic adsorption-desorption method,the content of total saponins in RADIX ASTRAGALI was determined to reveal the optimum conditions for separating and purifying total saponins from RADIX ASTRAGALI with macroporous resin.[Result] The optimum conditions for separation and purification of total saponins with AB-8 were as follows:extract concentration of 0.1 mg/ml,sampling volume of 3 BV,flow rate of 1.0 ml/min,washing with 4 BV of distilled water first and then desorption with 4 BV of 50% ethanol.Under these conditions,the yield of total saponins was reached as high as 52.1%.[Conclusion] This method is simple and feasible,which could meet industrial requirements. [Objective] The aim was to find out the optimum conditions for separation and purification of total saponins from RADIX ASTRAGALI. [Method] Employing ultraviolet spectrophotometry as dynamic adsorption-desorption method, the content of total saponins in RADIX ASTRAGALI was determined to reveal the optimum conditions for separating and purifying total saponins from RADIX ASTRAGALI with macroporous resin. [Result] The optimum conditions for separation and purification of total saponins with AB-8 were as follows: extract concentration of 0.1 mg / ml, sampling volume of 3 BV, flow rate of 1.0 ml / min, washing with 4 BV of distilled water first and then desorption with 4 BV of 50% ethanol.Under these conditions, the yield of total saponins was reached as high as 52.1%. [Conclusion] This method is simple and feasible, which could meet industrial requirements.