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在建立 6 羟多巴胺损伤的帕金森病大鼠模型基础上 ,应用放射配基结合分析法及Scanchard作图 ,测定不同时间点模型鼠及对照鼠尾壳核多巴胺D1和D2 受体的受体最大结合容量 (Bmax)和平衡解离常数 (KD)。结果显示 ,模型鼠毁损侧尾壳核多巴胺D2 受体Bmax显著升高 ,KD值显著降低 ,在 1个月模型组达到高峰。D1受体除了 2周模型组Bmax轻度升高、KD值轻度降低外 ,与对照组比较无差异。表明帕金森病模型鼠毁损侧尾壳核存在D2 受体上行调节 ,受体亲合力增高 ,D1受体活性无明显变化。
Based on establishing a rat model of Parkinson’s disease with 6-hydroxydopamine damage, radioligand binding assay and Scanchard plotting were used to determine the maximal receptors of dopamine D1 and D2 receptors in the putamen nucleus at different time points Binding capacity (Bmax) and equilibrium dissociation constant (KD). The results showed that the Bmax of dopamine D2 receptor in the caudate putamen was significantly increased and the KD was significantly decreased in model rats, which reached its peak at 1 month in model group. D1 receptor in the model group, except for 2 weeks slightly increased Bmax, KD slightly decreased, with no significant difference compared with the control group. The results showed that the upregulation of D2 receptors in the caudal putamen of Parkinson’s disease rats resulted in upregulation of D2 receptors, increased affinity of receptors, and no significant changes in D1 receptor activity.