目的探讨和厚朴酚(HNK)抑制9L胶质瘤细胞增殖并诱导其凋亡的作用。方法不同浓度HNK对9L胶质瘤细胞作用后,采用MTT实验评估9L细胞增殖水平,用细胞爬片Hoechst33258荧光染色及DNA Ladder检测细胞的凋亡情况,采用流式细胞术检测HNK对9L细胞周期的影响。结果 HNK呈剂量依赖性抑制9L细胞增殖,其48h的IC50为15μg/ml;HNK处理过的9L细胞,经Hoechst 33258染色后细胞核出现明显的凋亡形态学变化,并在DNA琼脂糖凝胶上可见特征性的细胞凋亡梯状条带;HNK阻滞9L细胞周期于G0/G1期。结论 HNK具有抑制9L胶质瘤细胞增殖并诱导其凋亡的作用。 Objective To investigate the effect of honokiol (HNK) on the proliferation and apoptosis of 9L glioma cells. Methods After treated with different concentration of HNK on 9L glioma cells, MTT assay was used to evaluate the proliferation of 9L cells. Hoechst33258 fluorescence staining and DNA ladder were used to detect the cell apoptosis. HNK was used to detect the cell cycle of 9L cells by flow cytometry Impact. Results HNK inhibited the proliferation of 9L cells in a dose-dependent manner. The IC50 of HNK at 48h was 15μg / ml. HNK-treated 9L cells showed obvious apoptotic morphological changes after Hoechst 33258 staining, and were detected on DNA agarose gel Characteristic apoptotic ladder cells can be seen; HNK block 9L cell cycle in G0 / G1 phase. Conclusion HNK can inhibit the proliferation of 9L glioma cells and induce its apoptosis.