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目的探讨白三烯受体拮抗剂对哮喘小鼠肺组织高迁移率族蛋白B1(HMGB1)表达的影响。方法72只SPF级Balb/c小鼠随机分为哮喘组(A组)、孟鲁司特治疗组(B组)和生理盐水对照组(C组),每组24只。建立哮喘模型。于第1、2、3、4周4个时段,HE染色观察肺组织病理改变,RT-PCR法检测肺组织HMGB1mRNA表达,免疫组化标记分析HMGB1在肺组织的分布。结果HE染色B组肺组织炎症较A组明显减轻。第2周时A、B组肺组织HMGB1的表达明显高于C组[(1.58±0.47)、(1.42±0.30)vs.(0.65±0.15)](P<0.01);B组肺组织HMGB1的表达随治疗时间的延长而减少。结论白三烯受体拮抗剂能抑制哮喘小鼠气道炎症,减少HMGB1的生成。
Objective To investigate the effect of leukotriene receptor antagonist on the expression of high mobility group box 1 (HMGB1) in lung tissue of asthmatic mice. Methods Seventy-two SPF Balb / c mice were randomly divided into asthma group (group A), montelukast treatment group (group B) and saline control group (group C), 24 rats in each group. Establish an asthma model. At 1, 2, 3 and 4 weeks, the pathological changes of lung tissue were observed by HE staining. The expression of HMGB1 mRNA in lung tissue was detected by RT-PCR and the distribution of HMGB1 in lung tissue by immunohistochemistry. Results Compared with group A, the inflammation of lung tissue in group B was significantly reduced. The expression of HMGB1 in group A and group B at the second week was significantly higher than that in group C [(1.58 ± 0.47), (1.42 ± 0.30) vs. (0.65 ± 0.15)] (P <0.01) Expression decreased with the extension of treatment time. Conclusion Leukotriene receptor antagonists can inhibit airway inflammation and reduce the formation of HMGB1 in asthmatic mice.