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目的:建立同时测定承德产及其他主产区北豆根中蝙蝠葛苏林碱和蝙蝠葛碱的含量及其HPLC-UV指纹图谱。方法:采用ZORBAX SB-C18(4.6 mm×250 mm,5μm)色谱柱,乙腈和-0.5%甲酸水溶剂系统梯度洗脱,检测波长为268 nm,流速1.0 mL.min-1,进样量10μL。结果:蝙蝠葛苏林碱和蝙蝠葛碱的线性范围分别为0.072～2.29 g.L-1(r=0.999 6),0.053～1.70 g.L-1(r=0.999 9);平均回收率分别为98.7%(RSD 1.5%),100.6%(RSD 1.9%);确立了承德产北豆根的指纹图谱共有9个共有峰,其他主产区的指纹图谱共有13个共有峰。指纹图谱方法重复性、精密度良好,承德产5批北豆根(原药材)除2号样品为0.404外其他样品均>0.9;其他主产区12批北豆根(饮片)除9号样品为0.716外其他样品均>0.9。结论:方法重复性好,专属性强,为北豆根药材的质量控制提供科学依据。
OBJECTIVE: To establish simultaneous determination of dauricine and dauricine in Chengde and other main producing areas by HPLC-UV fingerprinting. Methods: The mobile phase of ZORBAX SB-C18 (4.6 mm × 250 mm, 5 μm) was eluted with a gradient of acetonitrile and -0.5% formic acid in water. The detection wavelength was 268 nm, the flow rate was 1.0 mL · min- . RESULTS: The linear ranges of daurisoline and dauricine were 0.072-2.29 gL-1 (r = 0.999 6) and 0.053-1.70 gL-1 (r = 0.999 9) respectively. The average recoveries were 98.7% (RSD 1.5 %) And 100.6% (RSD 1.9%) respectively. There were 9 common peaks in the fingerprinting of Chengde yield, and 13 common peaks in other main producing areas. Fingerprinting method repeatability, good precision, Chengde 5 batches of North bean (raw material) in addition to 2 samples were 0.404 other samples were> 0.9; other main producing areas of 12 batches of North bean root (Pieces) except for the sample No. 9 0.716 other samples were> 0.9. Conclusion: The method has good repeatability and specificity, and provides a scientific basis for the quality control of Radix.