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The elongation of pollen tube is an importantprocess of sexual reproduction in higher plant. Cytoskeletonplays a major regulatory role in the elongation of pollentubes. But whether membrane skeleton is involved in thepollen tube elongation is not clear. In this study, immuno-chemical detection of spectrin-like protein has been carriedout in pollen tubes. By use of 2-dimensional electrophore-sis(2DE) and western blotting, two spectrin-like proteins arefound, one is 150 kD, and the other is 105 kD, with pI being4.54 and 4.39, respectively. 150 kD spectrin-like protein islocated in plasma membrane of pollen tube and 105 kDspectrin-like protein is located in cytoplasm, probably func-tioning as a subunit to form a dimmer (210 kD) in vivo. Theelongation of pollen tubes is inhibited after spectrin antibodywas injected into a growing pollen tube. These results suggestthat spectrin-like proteins exist in pollen tube and play animportant regulating role in the elongation process of pollentubes from lily.