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环磷酸腺苷(adenosine 3′,5′-cycliemonophosphate cAMP)作为细胞(包括淋巴细胞接受外界信息的第二信使,日趋受到免疫学工作者的重视。体内和体外实验表明:细胞内cAMP含量的变化可影响淋巴细胞的增殖和功能;抗原、有丝分裂原某些生物制剂(如胸腺素和转移因子等)以及细胞表面受体的配体作用于淋巴细胞时,均可导致细胞内cAMP含量的一时性升高。以上事实使得细胞内cAMP含量变化对淋巴细胞功能的调节作用成为近年来十分引人注意的问题。因此,淋巴细胞内cAMP含量的测定已较广泛地用作为研究淋巴细胞功能和鉴定免疫学生物制剂活性的指标之一。血细胞内cAMP含量的测定技术包括两个主要步骤。第一步是cAMP样品的制备,即
As a second messenger of lymphocytes receiving external information, adenosine 3 ’(5’-cycliemonophosphate cAMP) has drawn increasing attention from immunologists in vitro and in vivo and in vitro and has shown that changes of intracellular cAMP content Can affect the proliferation and function of lymphocytes; antigens, mitogen certain biological agents (such as thymosin and transfer factor, etc.) and the role of the ligand on the cell surface receptors in lymphocytes, can lead to temporary intracellular cAMP content Increased above the fact that changes in intracellular cAMP content on the regulation of lymphocyte function has become a very interesting issue in recent years.Therefore, the measurement of cAMP content in lymphocytes has been more widely used as a study of lymphocyte function and identification of immune One of the indicators of activity of biological agents.Capacitor determination of intracellular cAMP content consists of two main steps.The first step is the preparation of cAMP samples, namely