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目的:探讨细胞质转导肽-寡聚化结构域1-血凝素(cytoplasmic transduction peptide-oligomerization domain 1-hemagglutinin,CTP-OD1-HA)和CTP-OD2-HA融合肽对白血病K562细胞裸鼠皮下移植瘤的作用。方法:CTP-OD-HA(阳性对照)、CTP-HA(阴性对照)、PBS(空白对照)、CTP-OD1-HA和CTP-OD2-HA分别预处理K562细胞,并将其分别注射入裸鼠皮下,观察裸鼠皮下移植瘤的生长情况,TUNEL法检测移植瘤组织中肿瘤细胞的凋亡,免疫组织化学法检测肿瘤组织中凋亡相关蛋白Bax和Bcl-2的表达情况。结果:CTPOD1-HA和CTP-OD2-HA处理组移植瘤体积明显小于CTP-HA组(P<0.05),且肿瘤细胞内出现空泡样凋亡改变。TUNEL法检测结果显示,CTP-OD1-HA和CTP-OD2-HA处理组肿瘤细胞的凋亡改变比CTP-HA组更明显(P<0.05)。CTP-OD1-HA和CTP-OD2-HA处理组肿瘤组织中凋亡相关蛋白Bax蛋白的表达水平明显高于CTP-HA组(P<0.05),而Bcl-2蛋白的表达水平明显低于CTP-HA组(P<0.05)。结论:CTP-OD1-HA和CTP-OD2-HA融合肽能够抑制K562细胞在裸鼠体内的致瘤能力,并促进肿瘤细胞凋亡。
Objective: To investigate the effects of cytoplasmic transduction peptide-oligomerization domain 1-hemagglutinin (CTP-OD1-HA) and CTP-OD2-HA fusion peptide on the leukemia K562 cells in nude mice The role of xenografts. Methods: K562 cells were pretreated with CTP-OD-HA (positive control), CTP-HA (negative control), PBS (blank control), CTP-OD1-HA and CTP-OD2-HA, The growth of the subcutaneously transplanted tumor in nude mice was observed under the skin of mice. TUNEL method was used to detect the apoptosis of tumor cells in the transplanted tumor tissue. The expression of Bax and Bcl-2 were detected by immunohistochemistry. Results: The volume of transplanted tumor in CTPOD1-HA and CTP-OD2-HA treatment groups was significantly smaller than that in CTP-HA group (P <0.05), and vacuolar apoptosis was observed in tumor cells. The results of TUNEL assay showed that the apoptosis of tumor cells in CTP-OD1-HA and CTP-OD2-HA groups was more obvious than that in CTP-HA group (P <0.05). The expression of Bax protein in CTP-OD1-HA and CTP-OD2-HA groups was significantly higher than that in CTP-HA group (P <0.05), while the expression of Bcl-2 protein was significantly lower than that in CTP-HA group -HA group (P <0.05). Conclusion: The CTP-OD1-HA and CTP-OD2-HA fusion peptides can inhibit the tumorigenicity of K562 cells in nude mice and promote the apoptosis of tumor cells.