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目的分离、驯化和鉴定高效除铬(Ⅵ)菌株。方法从某电镀厂聚集区域的环境中采集样品,接种逐步升高铬(Ⅵ)浓度的培养基分离筛选出耐铬(Ⅵ)菌株,通过比较各菌株的除铬(Ⅵ)能力筛选出高效除铬(Ⅵ)菌株,并采用革兰氏染色、生理生化实验、16S r DNA等技术进行菌种鉴定。结果菌株CQMU-1的除铬效果最好;CQMU-1为革兰氏阴性球菌或短杆菌;能在500mg/L的含铬(Ⅵ)LB肉汤中生长,但生长受抑制;其最适生长p H为7.0~8.0,温度为20~35℃,好氧,化能异养,能利用大多数碳水化合物;经16S r DNA鉴定CQMU-1为产碱菌属。结论筛选出1株高效除铬(Ⅵ)菌株,可用于去除环境中铬(Ⅵ)污染。
Objective To isolate, acclimatize and identify highly efficient chromium (Ⅵ) - removing strains. Methods Chromium (Ⅵ) - resistant strains were isolated from the environment in the electroplating area and screened out by increasing the concentration of chromium (Ⅵ) in the plating solution. The efficiency of removal of chromium (Ⅵ) Chromium (Ⅵ) strain was identified by Gram stain, physiological and biochemical experiments, 16S r DNA and other techniques. Results The strain CQMU-1 had the best chromium removal effect. CQMU-1 was Gram-negative cocci or Brevibacterium. The strain CQMU-1 could grow in 500 mg / L chromium (Ⅵ) -containing LB broth but its growth was inhibited. Growth p H 7.0 ~ 8.0, temperature 20 ~ 35 ℃, aerobic, heterotrophic, can use most of the carbohydrates; identified by 16S r DNA CQMU-1 is Alcaligenes. Conclusion A strain of Chromium (Ⅵ) which is highly efficient is selected and can be used to remove chromium (Ⅵ) in the environment.