论文部分内容阅读
目的检测肺性脑病患者血清S-100β蛋白和神经元特异性烯醇酶(NSE)的浓度,探讨其临床意义。方法 29例健康者为健康对照组,慢性阻塞性肺疾病患者(COPD)无呼吸衰竭无肺性脑病26例,COPD并发呼吸衰竭35例,COPD并发肺性脑病38例。肺性脑病组分为轻型(14例)、中型(13例)、重型(11例)3组。用双抗体夹心酶联免疫吸附试验(ELISA)法检测血清中S-100β蛋白,用电化学发光法检测血清中NSE,并进行比较分析。结果 COPD并发肺性脑病组和COPD并发呼吸衰竭组血清S-100β和NSE值明显高于COPD组及健康对照组(P值均小于0.05),而COPD组与对照组血清S-100β和NSE值比较差异无统计学意义(P值均大于0.05)。肺性脑病重、中型组患者血清S-100β、NSE水平显著高于轻型组(P<0.05),重型和中型组差异无统计学意义(P>0.05)。结论检测血清S-100β蛋白和NSE浓度,对于COPD并发肺性脑病及呼吸衰竭的诊断、病情的评估及脑损害的程度具有重要临床意义,可作为评判颅内损伤的重要指标。
Objective To detect the serum concentration of S-100β protein and neuron-specific enolase (NSE) in patients with pulmonary encephalopathy and explore its clinical significance. Methods Twenty - nine healthy controls were included in the study. COPD patients had no pulmonary encephalopathy in 26 patients with respiratory failure, COPD complicated with respiratory failure in 35 patients, and COPD complicated with pulmonary encephalopathy in 38 patients. Pulmonary encephalopathy components were light (14 cases), medium (13 cases), heavy (11 cases) 3 groups. Serum S-100β protein was detected by double antibody sandwich enzyme-linked immunosorbent assay (ELISA), and the serum NSE was detected by electrochemiluminescence. Results The serum levels of S-100β and NSE in patients with COPD complicated by pulmonary encephalopathy and those with COPD complicated by respiratory failure were significantly higher than those in COPD patients and healthy controls (P <0.05). However, the serum levels of S-100β and NSE The difference was not statistically significant (P values were greater than 0.05). The levels of S-100β and NSE in patients with severe and moderate pulmonary encephalopathy were significantly higher than those in mild group (P <0.05), but no significant difference was found between severe and moderate group (P> 0.05). Conclusion The detection of serum S-100β protein and NSE concentration has important clinical significance for the diagnosis of COPD complicated with pulmonary encephalopathy and respiratory failure, the evaluation of the disease and the degree of brain damage. It can be used as an important index to judge the intracranial injury.