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应用扫描电镜研究了竹杆锈病病原菌(Stereostratumcorticioides)孢子的形成、病组织中菌丝分布和危害性。观察发现竹杆锈病病原菌的夏孢子开始产生时仅是产孢细胞顶端的囊状膨大,表面光滑无疣,随着夏孢子的发育成熟,表面开始出现疣状突起,最后发育成为小刺状突起。冬孢子堆中最初形成的一层冬孢子柄短而粗,以后产生的冬孢子柄细而长。冬孢子堆和夏孢子堆最初是埋生在角质层和1至2层表皮细胞下,最后突破表皮细胞层和角质层而外露。病组织中病原菌菌丝仅在寄主胞间隙中扩展,并不进入寄主细胞内,而仅以吸器形式进入寄主薄壁细胞和筛管中吸取营养。竹杆锈病的发生只能引起竹杆表皮细胞的坏死,并不能引起整株竹子枯死。而竹杆锈病的发生所造成的伤口却为后期病原菌提供了侵入和定殖的场所,这种后期病原菌的定殖和生长可能是导致病斑以上竹子枯死的主要原因。
The formation of spore of Stereostratum corticioides and the distribution and harmfulness of mycelium in diseased tissues were studied by SEM. It was observed that when the summer spores of the pathogen of the bamboo rod rust began to produce, only the top of the sporulation cells became swollen and the surface was smooth and warty. With the maturation of the summer spores, the surface began to appear verrucous and finally developed into small spines . The initial layer of teliospores formed in the winter spores is short and thick, and the teliospores produced later are thin and long. The teliospores and summer spores are initially embedded in the stratum corneum and 1 to 2 epidermal cells, eventually breaking through the epidermal cell layer and the stratum corneum. The diseased mycelium only spreads in the interstitial space of the host and does not enter the host cells, but absorbs nutrients only through the aspirate into the host parenchyma cells and the sieve tube. The occurrence of bamboo rust can only cause the death of bamboo pole epidermal cells, and can not cause the whole plant to die. However, the wound caused by the rust of bamboo pole provided the site for invasion and colonization of later pathogenic bacteria. The colonization and growth of this latter pathogen may be the main reason leading to the dead of the bamboo above the lesion.