丙型肝炎病毒 (ＨＣＶ)是一种单链正链ＲＮＡ病毒 ,可通过输血等方式传播 ,引起急、慢性肝炎。目前 ,ＨＣＶ的常规诊断方法主要有抗ＨＣＶ ＩｇＧ ,抗ＨＣＶ ＩｇＭ及ＨＣＶ ＲＮＡ的ＲＴ ＰＣＲ检测。对ＨＣＶ抗原的检测文献报道较少。我们利用基因工程表达的ＨＣＶＮＳ3蛋白 Hepatitis C virus (HCV) is a single-stranded positive-stranded RNA virus that can be transmitted by blood transfusion and cause acute and chronic hepatitis. At present, the routine diagnostic methods of HCV mainly include the detection of anti-HCV IgG, anti-HCV IgM and HCV RNA by RT PCR. The detection of HCV antigens less literature. We use genetically engineered HCV NS3 protein