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目的 探讨转化生长因子β1(transforming growth factor beta1,TGFβ1)及其受体(TGFbeta receptor, typeⅠand typeⅡ, TGFβRⅠ, TGFβRⅡ)在妊娠高血压综合征(妊高征)患者胎盘组织中的表达及其临床意义。 方法 采用免疫组化 SABC法检测 64 例妊高征患者(轻度 21 例,中度20例,重度23例)胎盘组织中TGFβ1、TGFβRⅠ、TGFβRⅡ的表达,20例正常孕妇为对照组。 结果 妊高征组胎盘绒毛滋养细胞及蜕膜细胞 TGFβ1,TGFβRⅠ,TGFβRⅡ的阳性表达率均明显高于对照组(TGFβ1: 70 31%和 35 00%,P< 0.01; TGFβRⅠ: 76 56%和 50 00%,P< 0.05; TGFβRⅡ:79 69%和45 00%,P<0.01)。妊高征组轻、中、重度患者TGFβ1、TGFβRⅠ和TGFβRⅡ表达的阳性率分别为:TGFβ1:52 38%、75 00%和 82 61%;TGFβRⅠ:57 14%、85 00%和 86 96%;TGFβRⅡ:61 90%、85 00%和91 30%,并随病情程度增加而升高。TGFβ1的表达与胎盘重量及新生儿体重呈负相关。 结论 胎盘TGFβ1及TGFβRⅠ、TGFβRⅡ表达异常引起滋养细胞浸润不足,可能是妊高征的发病因素之一。
Objective To investigate the expression of transforming growth factor β1 (TGFβ1) and its receptor (type Ⅰ and type Ⅱ, TGFβRⅠ, TGFβRⅡ) in placenta of patients with pregnancy induced hypertension (PIH) and its clinical significance . Methods Immunohistochemical SABC method was used to detect the expression of TGFβ1, TGFβRⅠ and TGFβRII in 64 cases of PIH (mild 21 cases, moderate 20 cases, severe 23 cases) and 20 normal pregnant women as the control group. Results The positive rates of TGFβ1, TGFβRⅠ, TGFβRⅡ in gestational trophoblastic placental trophoblastic cells and decidual cells were significantly higher than those in control group (TGFβ1: 70 31% and 35 00%, P <0.01; TGFβRI: 76 56% and 50 00%, P <0.05; TGFβRII: 79 69% and 45 00%, P <0.01). The positive rates of TGFβ1, TGFβRⅠ and TGFβRⅡ in PIH group were respectively TGFβ1: 52 38%, 75 00% and 82 61%; TGFβRⅠ: 57 14%, 85 00% and 86 96%; TGFβRⅡ: 61 90%, 85 00% and 91 30%, and increased with the severity of the disease. TGFβ1 expression was negatively correlated with placental weight and neonatal body weight. Conclusion The abnormal placental expression of TGFβ1, TGFβRⅠ and TGFβRⅡ may cause the infiltration of trophoblastic cells, which may be one of the pathogenesis of PIH.