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液体培养16至20h的里氏木霉（TrichodermaTeeseiQM9414）菌体，用0．9mol·L-1的氯化钾或20％的蔗糖为高渗缓冲液时，2．5g／L或5．0g／L质量浓度的纤维素酶和蜗牛酶混合液，30℃处理60～180Amin，可以获得稳定的原生质体。用50～55℃处理60min，或紫外线处理5min或0．1％碘乙酸30℃处理40min，原生质体全部失活。用0．03％～0．1％的中性红染色，钝化的原生质体全部着色，这是一种简便检测原生质体失活的新方法。
Liquid culture of Trichoderma reeseiQM9414 16-16h cells, with 0.9mol · L-1 potassium chloride or 20% sucrose as hypertonic buffer, 2.5g / L or 5.0g / L mass concentration of cellulase and snail enzyme mixture, 30 ° C for 60 ~ 180Amin, stable protoplasts can be obtained. With 50 ~ 55 ℃ treatment 60min, or UV treatment 5min or 0.1% iodoacetic acid 30 ℃ treatment 40min, protoplast inactivation. The passivated protoplasts were all colored with 0.03% to 0.1% neutral red, a new method for simply detecting inactivation of protoplasts.