目的了解浙江地区2010-2015年临床副溶血弧菌血清型分布及变迁规律,为该菌流行病学调查及疫情暴发早期预警提供基础数据。方法收集浙江省8家医院急性腹泻患者来源的副溶血弧菌,采用玻片凝集法进行血清学分型,分析各年份血清型的构成及变迁情况。结果 2010-2015年共收集副溶血弧菌890株,其中864株(97.1%)发生O血清凝集,分为9个O群;以O3(507株,57.0%)为主,其次为O4(266株,30.0%)和O1(51株,5.7%)。650株(73.0%)发生K血清凝集,分属于23种K型,居首位的为K6(474株,53.3%)。发生凝集的菌株中共有43种O、K血清型组合,其中O3∶K6型是主要流行的血清型,共472株,占53.0%。2010-2015年中除2013年以O4∶KUT型为主,占56.2%(73/130);其他年份均以O3∶K6型为主,所占比例依次为:52.7%、68.5%、60.5%、50.3%和63.7%。连续6年均有出现的血清型有6种,分别为∶O3∶K6、O4∶KUT、O4∶K8、O4∶K68、O1∶K36和O1∶KUT型;且2013-2015年连续3年中血清型O1∶K36、O4∶K8和O4∶K68每年的构成比在0.5%~3.0%之间,与2010-2012年连续3年的3.0%~15.0%相比明显有所下降(P<0.05)。结论浙江地区临床副溶血弧菌血清型分布较为广泛,以O3∶K6型为优势血清型。不同年份间存在一定的变异性。应加强对副溶血弧菌血清型的监测,为新血清型别的流行与暴发提供早期预警。 Objective To understand the distribution and variation of clinical serotypes of Vibrio parahaemolyticus from 2010 to 2015 in Zhejiang province and provide basic data for epidemiological investigation and early warning of epidemic outbreaks. Methods Vibrio parahaemolyticus was collected from 8 patients with acute diarrhea in Zhejiang Province. Serological typing was performed by slide agglutination method. The composition and changes of serotypes were analyzed. Results A total of 890 strains of Vibrio parahaemolyticus were collected from 2010 to 2015, of which 864 strains (97.1%) had O serum agglutination and were divided into 9 O groups. O3 (507 strains, 57.0%) was the major component, followed by O4 Strain, 30.0%) and O1 (51 strains, 5.7%). 650 strains (73.0%) developed K serum agglutination, which belonged to 23 K types, with K6 (474 ​​strains, 53.3%) ranking the top. There were 43 O and K serotypes in the agglutinated strains, of which O3: K6 was the predominant serotype, with a total of 472 strains (53.0%). In 2010-2015, the majority were O4: KUT types, accounting for 56.2% (73/130) except for 2013; O3: K6 was the main type in other years, accounting for 52.7%, 68.5% and 60.5% respectively, , 50.3% and 63.7% respectively. There were 6 serotypes in 6 consecutive years, which were: O3: K6, O4: KUT, O4: K8, O4: K68, O1: K36 and O1: KUT. The percentages of serotypes O1: K36, O4: K8 and O4: K68 in each year were between 0.5% and 3.0%, significantly decreased from 3.0% to 15.0% in three years from 2010 to 2012 (P <0.05 ). Conclusion The serotypes of Vibrio parahaemolyticus in Zhejiang Province are widely distributed, with O3: K6 as the predominant serotype. There are some variability in different years. Monitoring of Vibrio parahaemolyticus serotypes should be strengthened to provide early warning of the prevalence and outbreak of new serotypes.