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1991~1993年从长春等地采集的标本中分离得到Alternaria菌株。经过对病菌的致病性、形态、生长温度、pH范围及对不同碳、氮源的利用等项目的测定,确定该病菌为A.allernata(Fr.)Kcissler.是引起烟草赤星病的病原菌。病菌在5~35℃均可生长,但以25℃,4h条件下萌发率可达100%,病菌在9种不同的培养基上生长,其小燕麦琼胶培养基、PDA、玉米粉琼胶培养基适合于菌丝生长,而蔗糖琼胶培养基最适合分生孢子的产生.在氮源的利用上,有机氮和无机氮均可利用。在碳源的利用上,以甘露糖、淀粉、乳糖中菌丝扩展较快,病菌在pH3.2~9.2之间均可生长,但以pH7时最合适。
Alternaria strains were isolated from specimens collected from Changchun and other places from 1991 to 1993. After the pathogenicity of bacteria, morphology, growth temperature, pH range and the determination of different carbon and nitrogen sources and other projects to determine the bacteria A. allernata (Fr.) Kcissler. Is caused by tobacco brown spot pathogen. The germs could grow at 5 ~ 35 ℃, but the germination rate could reach 100% under the conditions of 25 ℃ and 4h. The germs grew on 9 kinds of different mediums. The germs of small oat agar, PDA, corn agar The medium is suitable for mycelial growth, whereas sucrose agar is best suited for the production of conidia. In the use of nitrogen sources, organic nitrogen and inorganic nitrogen are available. In the utilization of carbon source, mannose, starch, lactose mycelium expansion faster, the bacteria can grow between pH3.2 ~ 9.2, but the most suitable pH7.