论文部分内容阅读
将抑癌基因 ｐ1 6通过腺病毒载体导入小鼠肾癌Ｒｅｎｃａ细胞株中 ,观察 ｐ1 6基因导入后肿瘤细胞的生物学行为 ,并采用ＥＬＩＳＡ法检测Ｒｅｎｃａ细胞中 ｐ1 6蛋白的动态分泌水平 ,为抑癌基因疗法提供实验依据。材料和方法 小鼠 ｐ1 6及ＬａｃＺ重组腺病毒载体ＡｄＰ1 6和Ａ
The tumor suppressor gene p1 6 was introduced into mouse renal cancer Renca cell line by adenoviral vector, the biological behavior of tumor cells after the introduction of p1 6 gene was observed, and the dynamic secretion level of p16 protein in Renca cells was detected by ELISA Suppressor gene therapy to provide experimental evidence. Materials and Methods Mouse p1 6 and LacZ recombinant adenovirus AdP1 6 and A