从正常人血浆中分离α_1T-糖蛋白(α_1T)使成免疫化学纯,对这种糖蛋白的部分氨基酸序列和糖链进行了测定,为此,用赖氨酰蛋白内切酶、V8蛋白酶、胰蛋白酶和溴化氰对某些肽进行测序以分析羧甲基化的α_1T及其N-末端序列,用化学方法测定了大量的氨基酸(460个氨基酸)残基,并与其它蛋白质的肽序列进行了比较,发现其与白蛋白家族的蛋白质具有高度的同源性,而且,这个蛋白质家族的新成员——人α-白蛋白,显示出与α_1T的氨基酸序列完全相同,表明这两种蛋白质在氨基酸序列和 The α_1T-glycoprotein (α_1T) was separated from normal human plasma to be immunochemically pure, and the partial amino acid sequence and sugar chain of the glycoprotein were determined. For this purpose, lysyl endopeptidase, V8 protease, Some peptides were sequenced by trypsin and cyanogen bromide to analyze the carboxymethylated alpha 1 T and its N-terminal sequence and a large number of amino acid (460 amino acid) residues were chemically determined and compared to the peptide sequences of other proteins Were found to have a high degree of homology to the albumin family of proteins and that the new member of this protein family, human alpha-albumin, showed exactly the same amino acid sequence as alpha-1T, indicating that the two proteins In the amino acid sequence and