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目的 研究瘤内注射携带人内皮抑素 (hEN)基因的SA脂质体对裸鼠体内大肠癌LoVo细胞生长的抑制作用。方法 克隆、修饰hEN并构建真核表达质粒pcDNA3 hEN ,2 0 μg质粒加 40 μgSA脂质体构建SA pcDNA3hEN。 3 2只裸鼠背部皮下注射 2× 10 5/mlLoVo细胞悬液建立人大肠癌裸鼠模型。随机分 4组分别瘤内注射NS、SA、SA pcDNA3和SA pcDNA3hEN后 4、8、12、16d测量肿瘤体积。结果 SA pcDNA3hEN组肿瘤平均体积均小于对照组 ,第 12、16天的平均体积分别为 (0 .2 7± 0 .2 1)cm3 及 (0 .5 7± 0 .41)cm3 ,而对照组肿瘤体积为 :NS组 (0 .89± 0 .3 9)cm3及 (2 .5 0± 0 .91)cm3 ;SA组 :(0 .98± 0 .69)cm3 及 (1.98± 0 .5 9)cm3 ;SA pcDNA3组 :(0 .90± 0 .3 4)cm3 及 (1.88± 1.0 2 )cm3 ,差异有显著性 (P <0 .0 5 )。结论 瘤内注射携带hEN基因的SA脂质体可抑制裸鼠体内种植性人大肠癌的生长。
Objective To study the inhibitory effect of SA liposomes carrying human endostatin (hEN) gene on the growth of colorectal cancer LoVo cells in nude mice. Methods The hEN gene was cloned, modified and cloned into eukaryotic expression plasmid pcDNA3 hEN. 20 μg plasmid and 40 μg SA liposome were used to construct SA pcDNA3 hEN. Three nude mice were subcutaneously injected with 2 × 10 5 / ml LoVo cell suspension to establish a human colorectal cancer nude mouse model. Tumor volume was measured at 4, 8, 12 and 16 days after intratumoral injection of NS, SA, SA pcDNA3 and SA pcDNA3hEN respectively. Results The mean tumor volume of SA pcDNA3hEN group was smaller than that of the control group, with mean volume of (0.227 ± 0.21) cm3 and (0.57 ± 0.41) cm3 on the 12th and 16th days, respectively. While in the control group The tumor volume was (0.89 ± 0.39) cm3 and (2.50 ± 0.91) cm3 in the NS group, (0.98 ± 0.669 cm3) and (1.98 ± 0.5 9) cm3; SA pcDNA3 group: (0.90 ± 0.34) cm3 and (1.88 ± 1.02) cm3, the difference was significant (P <0.05). Conclusions Intrathecal injection of SA liposomes carrying hEN gene can inhibit the growth of human colon cancer in nude mice.