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目的 了解非灰髓炎肠道病毒 (EV)感染在婴幼儿急性下呼吸道感染 (ALRI)中的发病情况、临床特点及诊断方法。方法 ①采用通用引物RT -PCR及病毒分离检测全年住院ALRI婴幼儿鼻咽分泌物 (NPS)中的EV。②并用APAAP法检测RSV、IFV、PIV和AdV ,以资比较。结果 ①观察组 87例NPS中RT -PCR阳性 40例 ,EV分离阳性 2 6例。②阳性病例中同时检出其它呼吸道病毒 2 6例。结论 ①近半数住院ALRI婴幼儿存在EV感染 ,且常与其它呼吸道病毒混合感染。②EV -RT -PCR较EV分离敏感、实用。③EV感染无临床特异性
Objective To investigate the incidence, clinical features and diagnosis of non-myelitis enterovirus (EV) infection in acute lower respiratory tract infection (ALRI) in infants and young children. Methods ① The universal primers RT-PCR and virus isolation were used to detect the EV in infant nasopharyngeal secretions (NPS) during the year. ② APAAP method and the detection of RSV, IFV, PIV and AdV, for comparison. Results ① In the observation group, there were 40 RT-PCR positive cases in 87 cases of NPS and 26 cases positive EV separation. ② positive cases were detected at the same time other respiratory virus 26 cases. Conclusion ① Nearly half of hospitalized children with ALRI have EV infection and are often mixed with other respiratory viruses. ② EV-RT-PCR is more sensitive than EV separation, practical. ③EV infection is not clinically specific