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目的:建立了新型“香料”毒品中2种合成大麻素(CP47,497和其同系物CP47,497(C8))的液相色谱分析方法。方法:以甲醇作为提取溶剂。采用岛津ODS-SP型(250mm×4.6mm,5μm)色谱柱,以215nm作为检测波长。以(甲醇-乙腈)-高纯水为流动相进行梯度洗脱,流速1.2mL/min,柱温35℃,进样量10μL。结果:在优化条件下,CP47,497和CP47,497(C8)在1~100μg/mL范围内线性良好,相关系数均大于0.9999。方法的检出限(S/N≥3.3)为0.37μg/mL和0.49μg/mL。日内精密度(相对标准偏差(RSD))为0.82%~1.32%,日间精密度为1.46%~2.11%。CP47,497和CP47,497(C8)样品溶液在室温下60h内稳定性良好。结论:方法快速、灵敏、准确、可靠,适用于新型“香料”毒品样本中CP47,497和CP47,497(C8)的定量测定。
OBJECTIVE: To establish a liquid chromatographic method for the determination of two synthetic cannabinoids (CP47,497 and their homologues CP47,497 (C8)) in a new type of “spice” drug. Methods: Methanol was used as extraction solvent. Shimadzu ODS-SP type (250mm × 4.6mm, 5μm) column, with 215nm as the detection wavelength. The gradient elution was performed with (methanol - acetonitrile) - high purity water as the mobile phase at a flow rate of 1.2 mL / min. The column temperature was 35 ℃ and the injection volume was 10 μL. Results: Under the optimal conditions, the linearities of CP47, 497 and CP47, 497 (C8) in the range of 1 ~ 100μg / mL were good with the correlation coefficients greater than 0.9999. The limits of detection (S / N≥3.3) of the method were 0.37μg / mL and 0.49μg / mL. The intraday precision (relative standard deviation (RSD)) was 0.82% -1.32%, and the intraday precision was 1.46% -2.11%. The CP47, 497 and CP47, 497 (C8) sample solutions showed good stability within 60 h at room temperature. Conclusion: The method is rapid, sensitive, accurate and reliable and is suitable for the quantitative determination of CP47, 497 and CP47, 497 (C8) in new spice samples.