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为了研究TGFβ1在严重急性呼吸综合征(Severeacuterespiratorysyndrome,SARS)尸检肺组织中的表达情况及其在患者肺组织损伤中的可能作用,对2例SARS尸检肺组织进行病理学观察;应用免疫组化方法检测TGFβ1在尸检肺组织及对照肺组织中的表达情况,并进行半定量分析。结果显示病例一尸检肺组织主要病理改变为弥漫性肺泡损伤,透明膜形成及渗出性炎症。病例二尸检肺组织除了上述改变外,还伴有肺泡间质纤维增生和肺泡早期纤维化等机化性肺炎改变。TGFβ1平均灰度值在SARS患者肺组织为103.43±0.62;小叶性肺炎组织为131.47±2.64;正常肺组织中为144.24±0.09。3组比较有显著差别(P值<0.05)。SARS病毒感染后可引起急性肺间质和肺泡渗出性炎症,中后期病例还伴有肺泡间质纤维增生和肺泡早期纤维化;SARS患者肺组织损伤及纤维化与SARS冠状病毒感染后TGFβ1表达增强有关,提示抗TGFβ1治疗在SARS患者肺损伤、纤维化的预防、治疗过程中可能具有一定的临床意义。
In order to investigate the expression of TGFβ1 in the lung tissue of autopsy patients with severe acute respiratory syndrome (SARS) and its possible role in the lung injury, two cases of SARS were stained for pathology. The immunohistochemistry The expression of TGFβ1 in autopsy lung tissue and control lung tissue was detected and semi-quantitative analysis was performed. The results showed that a case of an autopsy of lung tissue pathological changes of diffuse alveolar damage, the formation of transparent membrane and exudative inflammation. Case two autopsy lung tissue in addition to the above changes, but also accompanied by alveolar interstitial fibrosis and early alveolar fibrosis and other opportunistic pneumonia changes. The average gray value of TGFβ1 in SARS patients was 103.43 ± 0.62 in lung tissue, 131.47 ± 2.64 in lobular pneumonia group and 144.24 ± 0.09.3 in normal lung tissue (P <0.05). SARS virus infection can cause acute lung interstitial and alveolar exudative inflammation, mid-and late-stage cases also associated with alveolar interstitial fibrosis and early alveolar fibrosis; SARS patients with lung injury and fibrosis and SARS coronavirus infection TGFβ1 expression Enhanced, suggesting that anti-TGFβ1 treatment in patients with lung injury lung injury, fibrosis prevention and treatment process may have some clinical significance.