论文部分内容阅读
目的建立HPLC双波长法同时测定红花药材中羟基红花黄色素A、芦丁、槲皮素和山柰酚含量的方法,为红花质量标准的研究提供科学依据。方法采用Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm)柱,以0.4%甲酸溶液为流动相A,以乙腈为流动相B,梯度洗脱,流速为0.8 ml·min-1,柱温为25℃,检测波长分别为360 nm,403 nm,进样量20μl。结果在上述色谱条件下,羟基红花黄色素A、芦丁、槲皮素和山柰酚分别在0.01~100.00μg·ml-1(r=1.000 0),0.01~10.00μg·ml-1(r≥0.998 5),0.01~10.00μg·ml-1(r≥0.998 5),0.01~10.00μg·ml-1(r≥0.997 5)线性关系良好。平均回收率羟基红花黄色素A为95.1%,芦丁为95.7%,槲皮素为95.2%,山柰酚为95.5%。结论该方法操作简便、重现性好、灵敏度高、结果准确,为控制红花药材质量提供了可靠的方法。
OBJECTIVE To establish a method for the simultaneous determination of hydroxysafflor yellow A, rutin, quercetin and kaempferol in Safflower Rhizoma by HPLC dual wavelength method, and to provide a scientific basis for the study of Safflower quality standard. Methods The mobile phase A was consisted of 0.4% formic acid solution and mobile phase B using acetonitrile as the mobile phase B on a Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm) with a gradient of 0.8 ml · min-1. The column temperature At 25 ℃, the detection wavelength was 360 nm and 403 nm, respectively. The injection volume was 20 μl. Results Under the above chromatographic conditions, the concentrations of hydroxysafflor yellow A, rutin, quercetin and kaempferol were respectively 0.01-100.00μg · ml-1 (r = 1.000 0) and 0.01-10.00μg · ml-1 0.998 5), 0.01 to 10.00 μg · ml-1 (r ≧ 0.998 5), and 0.01 to 10.00 μg · ml-1 (r ≧ 0.997 5). The average recovery was 95.1% for hydroxy-safflower A, 95.7% for rutin, 95.2% for quercetin and 95.5% for kaempferol. Conclusion The method is simple, reproducible, sensitive and accurate. It provides a reliable method for controlling the quality of safflower medicinal materials.