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目的:分析比较MEM培养基对带状疱疹减毒活疫苗卡那霉素残留量的影响。方法:分别使用海克隆、日水2种MEM培养基培养2BS细胞,经传代、感染、洗涤、收获、合并、冻干等工序制备带状疱疹减毒活疫苗,分析比较MEM培养基对带状疱疹减毒疫苗卡那霉素残留量的影响。结果:日水MEM培养基制备的带状疱疹减毒活疫苗卡那霉素残留量一般是海克隆MEM培养基制备疫苗的5-10倍。结论:美国海克隆实验公司的MEM培养基卡那霉素残留量明显小于日水MEM培养基。
Objective: To analyze and compare the effect of MEM medium on the kanamycin residues in live attenuated herpes zoster vaccine. Methods: 2BS cells were cultured in two kinds of MEM culture mediums, respectively. The attenuated live attenuated zoster vaccine was prepared by passage, infection, washing, harvesting, merging, Effect of Herpes Attenuated Vaccine Kanamycin Residues. Results: Residual amount of kanamycin in live attenuated herpes zoster vaccine prepared in daily water MEM culture medium was generally 5-10 times higher than that in the preparation of the vaccine by using Hepatic MEM medium. CONCLUSION: The residual amount of kanamycin in MEM culture medium of American clone laboratory was significantly lower than that of Japanese water MEM medium.