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用不同浓度秋水仙素处理美洲黑杨(Populus deltoides)与小叶杨(Populus simonii)杂种后代以诱导产生多倍体,并采用流式细胞术(FCM)对初选的多倍体材料进行DNA含量分析。结果表明:小于0.1%秋水仙素浓度处理对诱导多倍体无作用;0.3%及其以上浓度处理对诱导产生多倍体效果较好,但随处理浓度增加秋水仙素对杨树的毒害作用增大,0.5%浓度处理可使顶芽死亡率高达71.43%,且在顶芽死亡的植株中未发现多倍体单株。应用气孔性状对388株扦插苗进行多倍体初选后,采用流式细胞术对初选的5株拟多倍体进行DNA含量分析,鉴定到2株四倍体材料。获得的四倍体材料与二倍体植株在株高和叶绿素含量上差异显著,但在SOD和POD活性上无显著性差异。
Different progeny of Populus deltoides and Populus simonii hybrids were treated with colchicine at different concentrations to induce polyploidy. The primary polyploidy material was subjected to flow cytometry (FCM) for DNA content analysis. The results showed that the colchicine concentration of less than 0.1% had no effect on the induced polyploid; the treatment of 0.3% and above had good effect on inducing the polyploidy, but the toxic effect of colchicine on the poplar was increased with the treatment concentration The treatment of 0.5% concentration could make the death rate of terminal buds reach as high as 71.43%, and no polyploid plants were found in the plants with dead buds. After the primary polyploidy of 388 cuttings, the DNA content of the five primates was analyzed by flow cytometry. Two tetraploid materials were identified. The obtained tetraploid materials and diploid plants showed significant differences in plant height and chlorophyll content, but there was no significant difference in the activities of SOD and POD.