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目的研究华蟾素对人结肠癌细胞株SW480细胞生物学行为的影响。方法采用华蟾素(0.01mg/L、0.1mg/L、1mg/L)干预SW480细胞,MTT实验检测细胞增殖能力的改变,流式细胞术检测细胞凋亡比例变化,Transwell小室实验观察药物作用前后细胞侵袭能力的改变。结果华蟾素对SW480细胞有显著的增殖抑制作用,且呈现浓度和时间依赖性,经药物作用的细胞凋亡比例显著增高,侵袭能力明显下降。结论华蟾素能抑制结肠癌细胞增殖,诱导其凋亡,具有一定的临床应用前景。
Objective To study the effect of cinobufacini on the biological behavior of human colon cancer cell line SW480. Methods SW480 cells were treated with cinobufacini (0.01mg / L, 0.1mg / L, 1mg / L), the cell proliferation was detected by MTT assay, the percentage of apoptotic cells was detected by flow cytometry, and the effects of drugs were observed by Transwell chamber Changes in cell invasive ability before and after. Results Cinobufacini significantly inhibited the proliferation of SW480 cells in a time-and concentration-dependent manner. The percentage of apoptotic cells was significantly increased and the invasive ability was significantly decreased. Conclusion Cinobufacini can inhibit the proliferation of colon cancer cells and induce its apoptosis, which has some clinical application prospects.