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丁醇在分离提取的大鼠肝微粒体中，与微粒体中的细胞色素Ｐ４５０ⅡＥ１酶及加入的还原性辅酶Ⅱ（ＮＡＤＰＨ）在特定的温度下作用一定时间后，转化为丁醛。丁醇转化为丁醛的速率可作为评价细胞色素Ｐ４５０ⅡＥ１酶活性的指标。采用顶空气相色谱法可测定丁醇在微粒体中转化产生丁醛的含量。方法的检测限为０．７μｍｏｌ／Ｌ，变异系数８．１％～９．３％，回收率８５．３％。其干扰少、灵敏、快速、准确。方法的建立为间接测定细胞色素Ｐ４５０ⅡＥ１酶活性提供了可靠的测试手段
Butanol in the isolated rat liver microsomes, and the microsomal cytochrome P450 Ⅱ E1 enzyme and added reductive coenzyme Ⅱ (NADPH) at a specific temperature for a certain period of time, converted to butyraldehyde. The rate of conversion of butanol to butyraldehyde can be used as an indicator of cytochrome P450IIE1 enzyme activity. Headspace gas chromatography was used to determine the amount of butyraldehyde produced by the conversion of butanol to microsomes. The detection limit of the method was 0.7μmol / L, the coefficient of variation was 8.1% ~ 9.3% and the recovery rate was 85.3%. Its less interference, sensitive, fast and accurate. The establishment of the method provides a reliable test method for the indirect determination of cytochrome P450IIE1 activity