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目的：观察脊髓损伤（ＳＣＩ）后细胞内Ｃａ２＋（［Ｃａ２＋］ｉ）的动态变化，探讨其与脊髓神经功能损害的关系。方法：Ａｌｅｎ＇ｓ法致伤大鼠脊髓，于伤后１、４、８、２４、７２和１６８小时，采用原子吸收光谱分析和Ｌａ３＋阻断技术测定伤段脊髓［Ｃａ２＋］ｉ含量，参照Ｋｏｎｒａｄ的方法记录脊髓运动诱发电位（ＭＥＰ），应用斜板试验评价大鼠的运动功能。结果：ＳＣＩ后伤段脊髓［Ｃａ２＋］ｉ显著升高（Ｐ值＜０．０５或０．０１），与脊髓ＭＥＰ的变化和大鼠运动功能的损害呈显著相关关系。结论：ＳＣＩ后，伤段脊髓［Ｃａ２＋］ｉ超载可能在ＳＣＩ的病理发展机制中有重要意义。
Objective: To observe the dynamic changes of intracellular Ca2 + ([Ca2 +] i) after spinal cord injury (SCI) and to explore its relationship with spinal cord nerve dysfunction. Methods: Spinal cord injury was induced by Alen’s method in rats. At 1, 4, 8, 24, 72 and 168 hours after injury, the contents of [Ca2 +] i in the injured spinal cord were measured by atomic absorption spectrometry and La3 + Methods The spinal motor evoked potentials (MEPs) were recorded and the motor function of rats was evaluated by using the inclined plate test. Results: The level of [Ca2 +] i was significantly increased in the injured spinal cord of SCI (P <0.05 or 0.01), which was significantly correlated with the changes of MEP in the spinal cord and the impairment of motor function in rats. Conclusion: The overload of [Ca2 +] i in injured spinal cord may play an important role in the pathogenesis of SCI after SCI.