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目的:利用发根农杆菌诱导绞股蓝,得到毛状根。方法:采用外植体共感染接种法诱导绞股蓝毛状根,研究了不同菌株、外植体类型、预(共)培养时间、菌液浓度、浸染时间、乙酰丁香酮(As)和除菌培养基类型等因素对转化率的影响。结果:利用A4菌浸染不经过预培养的叶片,当发根农杆菌浓度在OD 600值为0.8,浸染10 min,共培养2 d,100μmol/L As、MS+300 mg/L Cab为除菌培养基为绞股蓝毛状根诱导的最佳条件。结论:用外植体共感染法诱导出绞股蓝毛状根,并确定了最佳诱导条件。
OBJECTIVE: To induce hairy roots by using Agrobacterium rhizogenes to induce gynostemma. Methods: Hairy roots of Gynostemma pentaphyllum were induced by co-infection of explants. The effects of different strains, explant types, pre-co-culture time, bacterial concentration, dipping time, Base type and other factors on the conversion rate. Results: Agrobacterium tumefaciens was used to disseminate the leaves without pre-culture. When the Agrobacterium rhizogenes concentration was 0.8 at OD 600, 10 min in co-culture, 100 μmol / L As and MS + 300 mg / Medium was the best condition for the induction of hairy roots of Gynostemma. Conclusion: The hairy roots of Gynostemma pentaphyllum were induced by explant co-infection method and the best induction conditions were determined.