增殖细胞核抗原(PCNA)/周期素是一种仅在增殖细胞中合成和表达的核内36KDa 多肽,新近研究表明,PCNA/周期素为DNA 聚合酶δ的一种辅助蛋白。PCNA 特异性自身抗体存在于一部分系统性红斑狼疮患者血清中,人类PCNA 抗体已被用于检测慢性髓样白血病患者外周血的增殖细胞。本文作者利用先前制备的两株抗PCNA 单抗,借助免疫荧光和流式细胞器探测增殖细胞。 Proliferating cell nuclear antigen (PCNA) / Cyclin is a nuclear 36KDa polypeptide that is synthesized and expressed only in proliferating cells. Recent studies have shown that PCNA / cyclin is a helper protein of DNA polymerase delta. PCNA-specific autoantibodies are present in the serum of a subset of patients with systemic lupus erythematosus, and human PCNA antibodies have been used to detect proliferating cells in the peripheral blood of patients with chronic myeloid leukemia. The authors used two previously prepared anti-PCNA mAbs to detect proliferating cells by immunofluorescence and flow cytometry.