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为探索用人免疫活性细胞重建猪免疫系统，用以测试活体动态猪→人异种移植免疫反应性。将中国内江猪经化疗行免疫成分摧毁后随机分为对照组、腹腔组和静脉组，每组各5只。腹腔组和静脉组分别于免疫成分摧毁后7天经腹腔和静脉注入人骨髓及脾细胞悬液。结果显示对照组长期存活，腹腔组存活3～54天，静脉组存活15小时～60天。三组在给药后7天，外周血白细胞降至最低，为给药前的1％～10％，而血小板、血红蛋白变化不大。腹腔组和静脉组白细胞计数恢复较对照组缓慢，血小板、血红蛋白差别不大。输入人细胞后1天、7天、60天，腹腔组和静脉组外周血细胞流式细胞仪检测，离心涂片免疫组化染色，均发现人细胞存在。脾、肺中有人类细胞浸润。因此认为，对免疫抑制的猪植入人骨髓及牌细胞，可使人细胞在猪体内形成嵌合，有发生异种移植物抗宿主病的可能，可为研究猪-人异种移植免疫反应性提供更好的动物模型。移植细胞时经腹腔注射可避免对植入细胞的细胞毒作用，可能优于静脉注入。
In order to explore the use of human immunocompetent cells to reconstruct the pig immune system to test live-to-human xenograft immunoreactivity. The Neijiang pigs in Neijiang district were randomly divided into control group, peritoneal group and vein group after the immunization components were destroyed. Five rats in each group. Intraperitoneal and intravenous groups were injected intraperitoneally and intravenously into human bone marrow and spleen cell suspensions 7 days after the immunization components were destroyed. The results showed that the control group long-term survival, survival of the abdominal cavity group 3 to 54 days, vein group survival 15 hours to 60 days. Three groups 7 days after administration, peripheral white blood cells to a minimum, before the administration of 1% to 10%, while platelets, hemoglobin little change. Intraperitoneal group and venous group white blood cell count recovery than the control group, platelet, hemoglobin difference is not big. Human cells were found on day 1, day 7, and day 60 after injection of human cells by flow cytometry of peripheral blood cells in the abdominal cavity and vein groups, and immunohistochemical staining of the centrifugal smears. Spleen, human lung cell infiltration. Therefore, implanted immunosuppressed pigs into human bone marrow and card cells, the formation of human cells can be formed in the pig chimeric, the possibility of occurrence of xenograft-host disease, may be in order to study pig-human xenograft immune response to provide Better animal model. Intraperitoneal injection of transplanted cells avoids the cytotoxic effect on the implanted cells and may be superior to intravenous injection.