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目的评价体外不同条件下活性炭对阿维菌素的吸附效果。方法用人工胃液、人工肠液配制浓度为10、5和2.5 g/L的阿维菌素溶液,分别调至pH1.9和pH6.8,设活性炭干预组和对照组,于孵育10 min、30 min、1 h、2 h和4 h后离心,紫外可见分光光度法测上清液中阿维菌素浓度,计算阿维菌素随时间的残留率和活性炭对阿维菌素的吸附率。结果 (1)在人工胃液与肠液环境中,不同炭药比(活性炭与阿维菌素质量比)时活性炭对阿维菌素均具有良好的吸附作用,并且存在时间依赖性;各个检测时间点活性炭对阿维菌素的吸附率(Q)均随炭药比的增加而递增。(2)活性炭-阿维菌素复合物的稳定性较强,在胃肠排空时间内基本不发生解离现象。结论活性炭吸附阿维菌素效果良好,且与炭药比呈正相关。
Objective To evaluate the adsorption of abamectin by activated carbon under different conditions in vitro. Methods The avermectin solution with the concentration of 10, 5 and 2.5 g / L was prepared with artificial gastric juice and artificial intestinal juice and adjusted to pH1.9 and pH6.8 respectively. The activated carbon intervention group and the control group were incubated for 10 min, 30 min, 1 h, 2 h and 4 h after centrifugation. The concentration of abamectin in the supernatant was measured by UV-Vis spectrophotometry. The residual rate of abamectin with time and the adsorption rate of abamectin by activated carbon were calculated. Results (1) In the artificial gastric juice and intestinal fluid environment, activated carbon had good adsorption effect on avermectin with different ratios of carbon to drug (mass ratio of activated carbon and abamectin), and there was a time-dependent manner. At each detection time point The adsorption rate (Q) of activated carbon to avermectin increased with the increase of the ratio of carbon to drug. (2) Activated carbon - avermectin complex strong stability in the gastrointestinal emptying time basically did not dissociate. Conclusion Activated carbon adsorption avermectin good effect, and the ratio of carbon and medicine was positively correlated.