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目的 通过对骨愈合过程中内源性碱性成纤维细胞生长因子(basic fibroblast growthfactor,bFGF) 合成变化的观察,探讨其在此过程中的作用,以此加深对骨生理学的认识,同时为干预骨愈合提供理论依据.方法 选用SD大鼠并将其胫骨造成骨缺损,用免疫组织化学及原位杂交技术检测bFGF基因和蛋白质在骨痂组织成熟过程中不同时期的变化情况.结果 免疫组织化学染色显示, 从骨折后4 天~3 周内,bFGF染色阳性信号出现在成骨细胞的胞浆中,且骨膜内层的未分化间充质细胞亦染色阳性.原位杂交结果表明,在缺损区新生编织骨中的成骨细胞对bFGFm RNA 强表达,并随着时间的推移,缺损区中骨痂组织逐渐增多,编织骨逐渐被板层骨取代,而排列于其骨表面上的成骨细胞对bFGFmRNA 不表达或弱表达.骨膜的未分化间充质细胞对之有表达.结论 成骨细胞有合成bFGF的能力, 主要表现在骨组织形成早期, 并且在骨修复过程中对调节骨膜中未分化间充质细胞增殖和分化可能有重要作用“,”Objective To observe the changes of synthesization of basic fibroblast growth factor(bFGF) and discuss its role during bone healing process in order to improve the knowledge of bone physiology and explore the theory for intervening bone healing Methods The SD rats were chosen and their tibia were cut into incompletely bone defects as the experimented models The changes of gene and protein of bFGF within the bone callus were tested by immunohistochemistry and in situ hybridization techniques Results The immunohistochemistry results showed that the positive signals appeared in the cytoplasm of ostoblasts from 4 days to 3 weeks after bone injury, and the mesenchymal stem cells were also positively stained In situ hybridization study showed that the bFGF were intensively expressed in the osteoblasts within bone defects As the time passing by, the bone callus increased and the woven bone was replaced by lamellar bone, while the osteoblasts had no or weak expression of bFGF Conclusion Osteoblasts had the potential of synthesization of bFGF, especially in initial period of bone formation and might play an important role during the process of periosteum mesenchymal stem cells proliferation and differentiation