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目的 观察邻苯二酚类螯合剂 95 0 1、95 0 2、76 0 1(CBMIDA)对辐射引起大鼠肝线粒体损伤的保护作用 ,以探讨其防护核素内污染辐射损伤的可能性。方法 采用γ射线外照射方法 ,引起大鼠肝线粒体辐射损伤 ,给予不同剂量的螯合剂 ,观察其保护作用。以硫代巴比妥酸比色法测定脂质过氧化产物MDA含量 ;在 5 2 0nm波长测定反应体系的浊度变化 ,以反映线粒体肿胀损伤程度 ,并制备电镜切片。结果 95 0 1(5× 10 - 6 mol L)、95 0 2 (10 - 5 mol L)、76 0 1(10 - 5 mol L)能显著抑制 12 0Gyγ辐射所致肝线粒体MDA生成 ,95 0 1、76 0 1对辐射引起的线粒体肿胀也有明显的保护作用 ,95 0 1并能防止辐射所致线粒体形态的病理性损伤。作者并对此类螯合剂对大鼠肝线粒体辐射损伤的保护作用机理进行了初步探讨。结论 邻苯二酚类螯合剂 95 0 1、95 0 2、76 0 1(CBMIDA)对外照射引起大鼠肝线粒体损伤有明显的保护作用
Objective To investigate the protective effects of catechol chelator 95 0 1,95 0 2,76 0 1 (CBMIDA) on radiation-induced hepatic mitochondrial damage in rats and to explore the possibility of radiation-induced damage to radionuclides in rats. Methods Radiofrequency irradiation was used to induce the damage of rat liver mitochondria radiation. Different doses of chelating agents were given to observe their protective effects. The MDA content of lipid peroxidation product was measured by thiobarbituric acid colorimetric method. The turbidity change of the reaction system was measured at 520 nm wavelength to reflect the extent of mitochondrial swelling and the preparation of electron microscopy. Results 955 (10 - 5 mol L), 95 0 2 (10 - 5 mol L) and 95 0 1 (10 - 5 mol L) could significantly inhibit the MDA production of hepatic mitochondria induced by 12 GyGamma radiation, 1,760 0 1 also has a significant protective effect against radiation-induced mitochondrial swelling, 95 0 1 and prevents the pathological damage of mitochondria due to radiation. The authors also discussed the protective mechanism of such chelating agents on rat liver mitochondrial radiation injury. Conclusion Catechol chelator 95 0 1,95 0 2,76 0 1 (CBMIDA) has a significant protective effect on rat liver mitochondrial damage induced by external radiation