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为了从分子水平评估莫桑比克罗非鱼、荷那龙罗非鱼及其正交子代(莫桑比克罗非鱼♀×荷那龙罗非鱼)和反交♂子代(荷那龙罗非鱼♀×莫桑比克罗非鱼)的耐盐性能,利♂用RT-PCR方法研究了盐胁迫下这4种罗非鱼不同组织中(鳃、肾、垂体和肌肉)热休克蛋白70(HSP70)基因的表达规律。结果表明:在盐度为22条件胁迫下4种罗非鱼各组织中HSP70表达水平总体呈现先增加后降低的规律,24 h时达最大值,48 h时下降至初始水平;盐度提高至35后HSP70的表达无显著变化。但不同种罗非鱼及不同组织之间也存在差异,反交子代鳃中HSP70在整个盐胁迫过程中都未上调表达;在盐度为22条件胁迫下,正反交子代肾中HSP70基因表达量比父母本肾中HSP70更早达到峰值;反交子代垂体中HSP70表达量在0~48 h内持续缓慢增加,48 h时达最大值;莫桑比克罗非鱼肌肉中HSP70基因在盐度22条件胁迫下表达量迅速升高,且维持较高水平直至盐度35条件胁迫之后。结果表明:HSP70表达水平的高低与罗非鱼的耐盐能力和应激程度密切相关,其中盐胁迫下肾中HSP70的表达规律与以上4种罗非鱼的耐盐能力之间相关性较强,可以作为评价这4种罗非鱼耐盐能力的潜在分子指标。
In order to assess Mozambique’s tilapia at the molecular level, the genus Heliconia and its Orthogonal Offspring (Mozambique Tilapia 荷 × Helon Tilapia) and crossbred ♂ offspring × Mozambique tilapia), the effects of heat shock protein 70 (HSP70) gene expression in different tissues (gill, kidney, pituitary and muscle) of four species of tilapia under salt stress were studied by RT-PCR The law of expression. The results showed that the expression level of HSP70 increased firstly and then decreased in all the four tilapia tissues under the condition of salinity 22, reaching the maximum value at 24 h and then decreasing to the initial level at 48 h. The salinity increased to 35 after HSP70 expression no significant change. However, there were also differences between different species of tilapia and different tissues. The expression of HSP70 in the gill of anti-hybrids was not up-regulated during the whole salt stress. Under the condition of salinity of 22, The expression level of HSP70 reached its peak earlier than that of HSP70 in the kidney of parents. The expression of HSP70 in pituitary of anti-offspring continued to increase slowly within 0-48 h and reached the maximum at 48 h. The expression of HSP70 in tilapia muscle of Mozambique Under the condition of degree 22, the expression level increased rapidly and maintained at a high level until the salinity 35 condition. The results showed that the expression level of HSP70 was closely related to the salt tolerance and stress level of Tilapia. The expression of HSP70 in kidney under salt stress was significantly correlated with the salt tolerance of the above four species of tilapia , Which can be used as a potential molecular index to evaluate the salt tolerance of the four species of tilapia.