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作者应用白细胞移动抑制试验,研究了几种肝胆疾病患者对正常人体胆汁蛋白成分的细胞免疫反应。采取死于非胃肠道疾患病人的胆汁,经磷酸盐缓冲液透析、超速离心去沉渣,上清部份再经硫酸铵沉淀、离心得上清液再经处理后通过Sepharose6B过柱、得第二峰经分离液用超滤浓缩制备成胆汁蛋白作白细胞移动试验的刺激原。白细胞移动抑制试验系采用毛细管法。测得30名健康人的平均移动指数为0.91,下限值为0.78(平均值减2个标准差,低于0.78者为显著抑制。检测了37例原发性胆汁性肝硬化(PBC)、27例慢性活动性肝炎(CAH)、10例硬化性胆管炎、11例肝外胆汁淤滞
The authors applied the leukocyte migration inhibition test to study the cellular immune responses to bile protein components in several human hepatobiliary diseases. Take the bile died of patients with non-gastrointestinal disorders, dialysis with phosphate buffer, ultracentrifugation to sediment, the supernatant and then ammonium sulfate precipitation, the supernatant was centrifuged and then through the Sepharose6B column, the first Erfeng separation by ultrafiltration concentrate prepared bile protein for leukocyte migration test stimulus. Leukocyte migration inhibition test using capillary method. The average movement index of 30 healthy people was 0.91 and the lower limit was 0.78 (mean minus 2 standard deviations, while those below 0.78 were significantly inhibited.) 37 cases of primary biliary cirrhosis (PBC) 27 cases of chronic active hepatitis (CAH), 10 cases of sclerosing cholangitis, 11 cases of extrahepatic cholestasis