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采用细胞内定位技术对cry1Ac基因进行修饰 ,其表达产物定位于内质网及衍生自内质网的蛋白体。通过基因枪法成功地将经过修饰的cry1Ac基因导入到优良杂交籼稻恢复系明恢 81中。对潮霉素抗性植株的PCR和Southern检测及ELISA分析证实 ,修饰的cry1Ac基因已整合到受体水稻品种中并得以表达 ,自交加代结合潮霉素筛选于T2 代获得转基因纯合系。抗虫性测试表明 ,部分转基因纯合系高抗二化螟 (Chilosuppressalis)。
The cry1Ac gene was modified by intracellular localization technology and its expression products were located in the endoplasmic reticulum and the endoplasmic reticulum-derived protein body. The modified cry1Ac gene was successfully introduced into Minghui 81, a fine indica rice restorer line by particle bombardment. PCR and Southern analysis of hygromycin resistant plants and ELISA analysis confirmed that the modified cry1Ac gene has been integrated into the recipient rice varieties and expressed, selfing plus hygromycin screening in T2 generation transgenic homozygotes. Insect resistance test showed that part of the transgenic homozygous high resistance Chilo suppressalis (Chilosuppressalis).