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目的了解舟山市桶装饮用水卫生情况。方法依据国标GB/T 8538—2008,对随机抽检的94份桶装饮用水,其中48份桶装饮用纯净水,46份桶装矿泉水进行铜绿假单胞菌的检测与鉴定。采用K-B纸片扩散法,对鉴定出的铜绿假单胞菌进行药敏试验。结果确认有3份水样检出铜绿假单胞菌,检出率为3.2%,其中桶装饮用纯净水检出3份,桶装天然矿泉水检出0份;3份阳性样品中共检测出4株铜绿假单胞菌,污染水平分别为27 cfu/250 ml、38 cfu/250 ml、6 cfu/250 ml、24 cfu/250 ml,其中3株产绿脓菌素,1株产荧光色素不产绿脓菌素。药敏试验结果显示:分离鉴定出的4株铜绿假单胞菌对16种抗生素中的新霉素(NEO)、恩诺沙星(ENR)、氨苄西林(AMP)、复方新诺明(SMZ)、青霉素(PNE)、强力霉素(DOX)6种抗生素均有抗性;对头孢曲松(CEFT)、环丙沙星(CIP)、氧氟沙星(OFL)、庆大霉素(GEN)、诺氟沙星(NOR)、头孢哌酮(CEF)等6种抗生素敏感。结论桶装饮用纯净水中存在铜绿假单胞菌污染,检出的铜绿假单胞菌具有多重耐药性。
Objective To understand the sanitary condition of bottled drinking water in Zhoushan City. Methods Based on the national standard GB / T 8538-2008, 94 samples of bottled drinking water were randomly selected, of which 48 samples were tested for drinking water and 46 samples were bottled mineral water for the detection and identification of Pseudomonas aeruginosa. The K-B disk diffusion method was used to detect the susceptibility of the identified Pseudomonas aeruginosa. The results confirmed that there were 3 water samples detected Pseudomonas aeruginosa, the detection rate was 3.2%, of which 3 were bottled purified water, bottled natural mineral water was detected 0; 3 positive samples were detected 4 strains of Pseudomonas aeruginosa, the pollution levels were 27 cfu / 250 ml, 38 cfu / 250 ml, 6 cfu / 250 ml and 24 cfu / 250 ml, respectively, of which 3 strains produced pyocyanin and 1 produced fluorescent pigment Not producing pyocyanin. The result of drug sensitivity test showed that four isolates of Pseudomonas aeruginosa were identified as neomycin (NEO), enrofloxacin (ENR), ampicillin (AMP), cotrimoxazole (SMZ , Penicillin (PNE) and doxorubicin (DOX). The resistance to ceftriaxone (CEFT), ciprofloxacin (CIP), OFL, GEN), norfloxacin (NOR) and cefoperazone (CEF). Conclusion Pseudomonas aeruginosa was found to be contaminated in bottled drinking pure water, and Pseudomonas aeruginosa was detected with multiple drug resistance.