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真菌信息素受体基因控制着真菌有性生殖过程,研究其序列组成的保守性和多态性对于认识真菌系统进化规律具有重要意义.利用简并引物从杨柳田头菇(Agrocybe salicacola)和茶树菇(A.aegerita)两种食用菌的16个野生菌株中扩增获得51条信息素受体基因片段.从各菌株中分别得到1-6条数量不等的片段,所有序列可分为两大类群.两种食用菌的少部分信息素受体基因序列聚在一起,大部分序列呈交错分布,显示出较远的亲缘关系和丰富的多态性.经翻译获得的氨基酸序列显示出更高的保守性,在最小进化树中,这些蛋白氨基酸序列可分为三大类群,茶树菇各菌株的氨基酸序列更明显地聚在一起,而且来自相近区域的菌株相似性最明显.基因邻接树和蛋白最小进化树分析都表明,真菌信息素受体基因的起源可能有两个.研究结果为真菌交配型基因研究提供了重要信息.
The fungal pheromone receptor gene controls the process of fungal sexual reproduction, and the conservation of its sequence composition and its polymorphism are of great significance for the understanding of the evolutionary rules of the fungal system.Degenerative primers were used to detect the fungal pathogen from Agrocybe salicacola and Camellia sinensis 51 pheromone receptor gene fragments were amplified from 16 wild strains of A. egerita and edible mushrooms.A total of 1-6 fragments ranging from 1 to 6 were obtained from each strain.These sequences were divided into two There were many pheromone receptor gene sequences of two kinds of edible fungi, most of the sequences were staggered, showing more distantly related and abundant polymorphisms.The amino acid sequences obtained by translation showed more In the smallest tree, the amino acid sequences of these proteins can be divided into three groups, and the amino acid sequences of each strain of Agrocybe aegerita gather together more prominently, and the similarity of the strains from the similar regions is the most obvious. And phylogenetic tree analysis indicated that there may be two origins of the fungal pheromone receptor gene.These results provide important information for the study of mating gene of fungi.