论文部分内容阅读
本文以水稻三系及杂种的花药为材料,考察了淀粉代谢酶类亏损、恢复与雄性不育的相关作用。测验表明,不育系花药含淀粉0.5μg/spl(小穗花药共6枚),只达可育株系含量的1/12至1/10,花药含蛋白质15μg/spl,相当于1/7—1/5可育株系含量.对比分析各株系发育中花药的α-,β-淀粉酶,P-酶,R酶活力,得出P-酶活力为0.3μgPi/spl·h,α-淀粉酶活力为1.2 IDC/spl·h.恢复后的杂种代花药P-酶活力上升5—8倍,α-淀粉酶活力上升2.5-5倍.凝胶电泳证明。P-酶从单核期,α-淀粉酶Ⅱ从双核期开始恢复。比较3种恢复系在杂种代的恢复程序,得出其恢复力顺序为IR 244-6≥IR 24》铁陆17.这正好是花粉可育率恢复力顺序.这些事实足以表明不育性与淀粉合成有关酶的亏损相关,育性恢复是多因量变现象.
In this paper, three lines of rice and hybrid anthers as material, investigated the loss of starch metabolism enzymes, and restore the role of male sterility. The results showed that male sterile anthers contained starch 0.5μg / spl (total of 6 spikelets anther), only 1/12 to 1/10 of that of fertile lines and 15μg / spl of anther protein, which corresponded to 1/7 -1/5 fertile lines.Analysis of α, β-amylase, P-enzyme and R enzyme activities of anthers in each line of development showed that P-enzyme activity was 0.3μgPi / spl · h, α --Amylase activity was 1.2 IDC / spl · h. After the recovery of the hybrid anthers P-enzyme activity increased 5-8 times, α-amylase activity increased 2.5-5 times. Gel electrophoresis proved. P-enzyme from the mononuclear phase, α-amylase Ⅱ recovery from the binuclear phase. The restoration programs of the three restorer lines on the hybrid generation were compared to find that the order of restoring force was IR 244-6≥IR 24 "Tielu 17. This is exactly the order of pollen fertility resilience. These facts are sufficient to show that infertility and Starch synthesis related to the loss of enzymes, fertility restoration is a multi-variable phenomenon.