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从毛桃[Prunus persica(Linn.)Batsch.]叶片中克隆了PpSnRK1蛋白激酶β亚基编码基因PpSnRK1β1和PpSnRK1β2,其c DNA全长分别为720 bp和867 bp。q RT-PCR组织表达分析表明,PpSnRK1β1在‘鲁星’桃叶片中的表达量最高,PpSnRK1β2在其果实中表达量最高,两者在其花中的表达量均较低。通过农杆菌介导转化番茄,获得超表达PpSnRK1β1的番茄株系T21、T23和超表达PpSnRK1β2的株系T91、T92。研究发现,与野生型(WT)相比,超表达株系T23和T91叶片的Sn RK1蛋白激酶活性分别增加9.84%和53.32%。T91叶片净光合速率比WT提高17.02%;叶片可溶性糖含量比WT增加34.00%。T91和T92叶片淀粉含量分别约是WT的2倍。T23、T91和T92果实维生素C含量分别比WT下降19.21%、38.49%和39.76%。因此说明PpSnRK1β1和PpSnRK1β2参与了光合作用过程,调控碳代谢及次生代谢过程。
PpSnRK1 protein kinase β subunit encoding genes PpSnRK1β1 and PpSnRK1β2 were cloned from leaves of Prunus persica (Linn.) Batsch. The full-length cDNAs of PpSnRK1 were 720 bp and 867 bp, respectively. q RT-PCR analysis showed that the expression level of PpSnRK1β1 was the highest in ’Lusing’ peach leaves, PpSnRK1β2 was the highest in the fruit, and both of them were lower in the flower. The tomato plants T21 and T23 overexpressing PpSnRK1β1 and T91 and T92 overexpressing PpSnRK1β2 were obtained by Agrobacterium tumefaciens transformation. The results showed that Sn RK1 protein kinase activity in T23 and T91 overexpression lines increased by 9.84% and 53.32%, respectively, compared with wild type (WT). The net photosynthetic rate of T91 leaves was increased by 17.02% compared with that of WT. The content of soluble sugar in leaves increased by 34.00% compared with WT. T91 and T92 leaf starch content is about twice WT. T23, T91 and T92 fruit vitamin C content decreased by 19.21%, 38.49% and 39.76% than WT respectively. Therefore, PpSnRK1β1 and PpSnRK1β2 are involved in the photosynthesis process and regulate carbon metabolism and secondary metabolism.