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目的观察全反式维甲酸(all-trans retinoic acid,ATRA)对肝癌细胞株CX26、CX32和 CX43基因表达的影响。方法体外培养肝癌细胞株SMMC-7721和BEL-7404。分别用常规培养基和含ATRA(10-5mol／L)的培养基培养两种细胞,在ATRA处理后的24、48、72 h提取细胞总mRNA。采用RT-PCR方法检测CX26、CX32、CK43基因的表达。结果用常规培养基培养的SMMC-7721细胞不表达CX26 mRNA和CX32 mRNA,但是经ATRA培养48 h开始出现CX26 mRNA的表达,经 ATRA培养72 h后出现CX32 mRNA的表达。常规培养的BEL-7404细胞没有CX26 mRNA和CX32 mRNA的表达,但是经ATRA培养48 h开始出现CX26 mRNA和CX32 mRNA的表达。两种肝癌细胞株在经ATRA处理前后均有CX43 mRNA的表达。结论全反式维甲酸能够在转录水平上调CX26、 CX32基因在肝癌细胞SMMC-7721和BEL-7404中的表达。
Objective To observe the effect of all-trans retinoic acid (ATRA) on the gene expression of CX26, CX32 and CX43 in hepatocellular carcinoma cell lines. Methods Hepatoma cell lines SMMC-7721 and BEL-7404 were cultured in vitro. The two kinds of cells were cultured with conventional medium and medium containing ATRA (10-5mol / L) respectively, and the total mRNA was extracted 24, 48 and 72 hours after ATRA treatment. The expression of CX26, CX32 and CK43 genes was detected by RT-PCR. Results CX26 mRNA and CX32 mRNA were not expressed in SMMC-7721 cells cultured in conventional medium. However, the expression of CX26 mRNA began to occur 48 h after ATRA treatment. The expression of CX32 mRNA was observed after 72 h of ATRA. Conventional cultured BEL-7404 cells did not express CX26 mRNA and CX32 mRNA but began to express CX26 mRNA and CX32 mRNA 48 hours after ATRA culture. The expression of CX43 mRNA in both hepatoma cell lines before and after ATRA treatment. Conclusion All-trans retinoic acid can upregulate the expression of CX26 and CX32 genes in hepatocellular carcinoma cells SMMC-7721 and BEL-7404 at the transcriptional level.