自Saiki首先应用聚合酶链反应(Polsamerase Chain reaction,PCR)检测镰状红细胞贫血病人以来,PCR技术在临床检测中得到日趋广泛的应用。PCR利用变性、退火及延伸的反复循环,可在体外将目的DNA片段迅速扩增百万倍,极大提高了检测方法的敏感性及特异性。近来PCR技术不断改进。我们采用简化PCR反应步骤,合并退火及延 Since Saiki first used the polymerase chain reaction (Polsamerase Chain Reaction, PCR) to detect patients with sickle cell disease, PCR technology has been increasingly used in clinical testing. PCR denaturation, annealing and extension of the repeated cycles, the target DNA fragment in vitro rapid amplification of millions of times, greatly improving the detection sensitivity and specificity. Recent PCR technology continues to improve. We used simplified PCR reaction steps, combined annealing and delay