论文部分内容阅读
利用已建立的受四环素调控ＬＭＰ1表达的鼻咽癌细胞系 ,用受ＣＭＶ启动子调控的ＮＦκＢ报道基因质粒ｐＮＦκＢ -ｌｕｃ的荧光素酶表达分析ＮＦκＢ的活性 ,并以核蛋白的Ｗｅｓｔｅｒｎ印迹方法观察ＬＭＰ1表达前后核内ＮＦκＢ组分ｐ65量的改变 ,用全蛋白Ｗｅｓｔｅｒｎ印迹
Using the established tetracycline-regulated LMP1-expressing nasopharyngeal carcinoma cell line, NFκB activity was analyzed by luciferase expression of the NFκB reporter gene plasmid pNFκB -luc regulated by the CMV promoter. LMP1 Before and after the expression of nuclear NFκB component p65 changes in the amount of protein with Western blot