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目的：：建立测定盐酸伐昔洛韦胶囊含量的HPLC法。方法：色谱柱为Inertsil ODS (250 mm ×4.6 mm,5μm),以甲醇-0.02 mol·L-1磷酸二氢钾溶液(20∶80)为流动相,流速为1.0 ml·min-1,检测波长为251 nm,柱温30℃,进样量20μl。结果：盐酸伐昔洛韦在2~40μg·ml-1浓度范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率为99.8%,RSD=0.16%(n=9)。结论：该检测方法简便、准确度高、专属性强,可用于盐酸伐昔洛韦胶囊含量测定。“,”Objective:To develop an HPLC method for the determination of valacyclovir hydrochloride capsules. Methods: An Inertsil ODS (250 mm × 4. 6 mm,5μm) column was employed with methanol-0. 02 mol·L-1 potassium dihydrogen phosphate solution (20∶80) as the mobile phase, the flow rate was 1. 0 ml·min-1, the detection wavelength was at 251 nm, the column temperature was 30℃,and the sample size was 20μl. Results:The linear range of valacyclovir hydrochloride was 2-40μg·ml-1 , the correlation coeffi-cient was 0. 999 9 and the average recovery was 99. 8%(RSD=0. 16%,n=9). Conclusion:The analytical method is simple, accu-rate and special, which can be used in the content determination and quality control of valacyclovir hydrochloride capsules.