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为了观察重组人干细胞因子 (rhSCF)和 (或 )粒细胞集落刺激因子 (rhG CSF)动员外周血干细胞的效果 ,本研究将 15只正常成年猕猴分成 3组 ,每天 1次 ,连续 14天分别皮下注射赋形剂、rhG CSF 10 μg/ (kg·d)和rhSCF5 0 μ/ (kg·d) +rhG CSF 10 μ/ (kg·d)。结果表明 ,单独给rhG CSF组的外周血白细胞数最高值在给药后第 7天出现 ,为给药前值的 4 11% ,其后很快下降。联合给药组外周血白细胞数最高值在第 9天出现 ,为给药前值的 5 38% ,第 7至第 9天的外周血白细胞数在 5 35 % - 5 38%之间。单独给药组外周血CFU GM最高值在给药后第 5天为给药前值的 937% ,联合给药组在第 9天为 1175 %。结论 :rhG CSF对外周血干 /祖细胞有明显的动员作用 ,rhSCF +rhG CSF联合动员的效果优于rhG CSF单用
In order to observe the effects of recombinant human stem cell factor (rhSCF) and / or granulocyte colony-stimulating factor (rhG CSF) mobilizing peripheral blood stem cells, 15 normal adult rhesus monkeys were divided into 3 groups, once daily for 14 consecutive days, respectively Injection vehicle, rhG CSF 10 μg / (kg · d) and rhSCF5 0 μ / (kg · d) + rhG CSF 10 μ / (kg · d). The results showed that the highest number of peripheral blood leukocytes in rhG CSF alone group appeared on the 7th day after administration, which was 41.1% of the value before administration, and then decreased rapidly thereafter. The highest number of peripheral blood leukocytes in the combination group appeared on day 9, which was 538% of the pre-administration value, and the number of peripheral leukocytes on days 7 to 9 was between 35.35% and 538%. The highest CFU GM in the peripheral blood of the monotherapy group was 937% of the pre-administration value on the fifth day after administration and 1175% on the ninth day in the combination administration group. Conclusion: rhG CSF has a significant mobilization effect on peripheral blood stem / progenitor cells. The combination of rhSCF + rhG CSF and mobilization is better than that of rhG CSF alone