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目的 探讨不同剂量人类胰岛素样生长因子 Ⅰ (IGF Ⅰ )对子宫内膜癌细胞系中孕激素受体亚型表达的动态变化的调控。方法 体外培养子宫内膜癌细胞系HEC IB ,以乳腺癌细胞系MCF 7为对照 ,采用蛋白印迹 (Westernblot)法观察不同剂量IGF Ⅰ对两种孕激素受体亚型表达的动态变化的调控。结果 (1)HEC IB细胞中 ,IGF Ⅰ 10ng/ml作用 2 4h ,人孕激素受体B亚型 (hPRB)表达有明显上调作用 ,随着剂量的增加、作用时间的延长 ,hPRB表达逐渐下调 ,IGF Ⅰ 2 0ng/ml作用 72h及 4 0ng/ml作用 4 8h时 ,下调最明显。人孕激素受体A亚型 (hPRA)表达的变化趋势与hPRB大致相同 ,IGF Ⅰ为 2 0ng/ml作用 4 8h时下调至最低。 (2 )MCF 7细胞中 ,IGF Ⅰ 10、4 0ng/ml对hPRA、hPRB表达均有上调的作用 ,在第 2 4、4 8、72h时较为明显。IGF Ⅰ 2 0ng/ml作用 2 4h对hPRB表达有上调的作用 ,但在 4 8、72h出现明显的下调 ;而对hPRA表达均为下调作用。结论 (1)IGF Ⅰ对人孕激素受体亚型的调控具有细胞特异性、以及时间和剂量的依赖性。 (2 )HEC IB细胞中 ,IGF Ⅰ 10ng/ml作用2 4h对hPRA、hPRB表达有上调作用 ,随着剂量的增加、作用时间的延长 ,hPRA、hPRB表达渐下调
Objective To investigate the regulation of the dynamic changes of progesterone receptor subtypes expression in human endometrial carcinoma cell lines with different doses of human insulin-like growth factor Ⅰ (IGF Ⅰ). Methods The endometrial carcinoma cell line HEC IB was cultured in vitro. The breast cancer cell line MCF 7 was used as a control. Western blotting was used to observe the effects of different doses of IGF Ⅰ on the dynamic changes of the two progesterone receptor subtypes. Results (1) The expression of human progesterone receptor subtype B (hPRB) was significantly up-regulated in HEC IB cells treated with 10 ng / ml IGF Ⅰ for 24 h, and the expression of hPRB was down-regulated with the increase of dose and the prolongation of action time , IGF Ⅰ 2 0ng / ml effect 72h and 4 0ng / ml 48h, the most significant down-regulation. The trend of human progesterone receptor A subtype (hPRA) expression was roughly the same as that of hPRB. IGF Ⅰ was reduced to the lowest level at 20 h / ml at 48 h. (2) The effect of IGF Ⅰ 10,4 0 ng / ml on the expression of hPRA and hPRB in MCF 7 cells was more obvious at the 2nd, 4th, 8th and 72th hour. The effect of IGF Ⅰ 2 0ng / ml for 24 h on hPRB expression was down-regulated, but down-regulated 48 h and 72 h, but down-regulated hPRA expression. Conclusion (1) IGF Ⅰ has the characteristics of cell-specific, time-and dose-dependent regulation of human progesterone receptor subtypes. (2) In HEC IB cells, the expression of hPRA and hPRB was up-regulated after treated with 10 ng / ml IGF Ⅰ for 24 h. With the increase of dose, the expression of hPRA and hPRB gradually decreased