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目的:应用苄基二甲基十四烷氯化铵(benzyldimethyltetrade-cylammoniumchloride,BAC)建立犬贲门失弛缓症动物模型.方法:将BAC4mmol/L12mL在胃镜下环周注入犬下食管括约肌(LES),用生理盐水作正常对照,注射6wk后测定LES的压力以及LES的松弛度.观察两组动物临床表现,食管吞钡时食管钡剂潴留情况.应用免疫组化(ABC法)染色LES组织观察NOS神经元的差异.结果:BAC处理组LES压力显著高于生理盐水组(5.66±0.56kpavs3.03±0.69kpa,P<0.01),LES的松弛率和松弛度明显低于生理盐水组(松弛率:40%vs100%;松弛度:13.1±3.9%vs94.3±3.4%),差别十分显著(P<0.01),反食为60%vs0%(P<0.01)质量减轻1.1±0.5kgvs-0.5±0.2kg(P<0.05).食管吞钡显示,BAC处理组食管钡剂明显潴留,而生理盐水组正常.免疫组化染色证实BAC处理组NOS阳性神经元明显减少.结论:LES局部应用BAC,能有效建立贲门失弛缓症犬动物模型.
OBJECTIVE: To establish animal model of achalasia in dogs with benzyldimethyltetrade-cylammonium chloride (BAC) .Methods: BAC4mmol / L 12mL was injected into the lower esophageal sphincter (LES) Normal saline was used as normal control, LES pressure and LES relaxation were measured 6 weeks after injection.Observation of clinical manifestations of the two groups of animals, esophageal barium esophageal barium retention when using immunohistochemistry (ABC method) stained LES tissue observed NOS Neurons.Results: The LES pressure in BAC treatment group was significantly higher than that in saline group (5.66 ± 0.56 kpa vs 3.03 ± 0.69 kPa, P <0.01), and relaxation and relaxation of LES were significantly lower than those in saline group (relaxation rate : 40% vs100%; sag: 13.1 ± 3.9% vs94.3 ± 3.4%), the difference was significant (P <0.01) and antifeedant was 60% vs0% ± 0.2kg (P <0.05) .Based on barium esophagealis, esophageal barium retention was observed in BAC group and normal saline group was normal.NOS positive neurons in BAC group were significantly decreased by immunohistochemical staining.Conclusion: Local application of BAC , Can effectively establish achalasia dog animal model.