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目的 :了解应用二甲基奶油黄 ( DAB)诱发大鼠肝癌过程中大鼠免疫功能变化 ,观察大鼠白细胞介素 2( IL- 2 )、淋巴细胞转化率改变及其相互关系。方法 :在诱发大鼠肝炎、肝硬化、肝癌 3个阶段分别取大鼠脾脏淋巴细胞 ,测定 IL- 2活性和淋巴细胞转化率。结果 :肝炎、肝硬化、肝癌期大鼠 IL- 2活性、淋巴细胞转化率降低 ( P <0 .0 5或 <0 .0 1)。对照、肝癌组大鼠 IL- 2与淋巴细胞转化率呈正相关 ( P <0 .0 5 ) ,肝炎、肝硬化组大鼠 IL- 2与淋巴细胞转化率无关 ( P >0 .0 5 )。结论 :诱发大鼠肝癌过程中 ,IL - 2活性、淋巴细胞转化率逐步降低 ,肝癌组大鼠 IL - 2活性降低与淋巴细胞合成功能下降有关。肝炎期 IL - 2活性显著下降提示有早期肝癌发生的可能
Objective : To understand the change of immune function in rat liver cancer induced by DAB and observe the changes of interleukin 2 (IL-2) and lymphocyte transformation and their relationship. METHODS: Rat splenic lymphocytes were obtained from three stages of hepatic inflammation, liver cirrhosis and hepatic carcinoma. IL-2 activity and lymphocyte transformation rate were measured. RESULTS: IL-2 activity and lymphocyte transformation rate in rats with hepatitis, liver cirrhosis, and liver cancer were decreased (P <0.05 or <0.01). There was a positive correlation between IL-2 and lymphocyte transformation rate in control and liver cancer rats (P < 0.05), and there was no relationship between IL-2 and lymphocyte transformation in rats with hepatitis and cirrhosis (P> 0.05). Conclusion : The activity of IL-2 and the lymphocyte transformation rate gradually decreased during the process of rat liver cancer induction. The decrease of IL-2 activity in the liver cancer group was related to the decrease of lymphocyte synthesis. Significant decline in IL-2 activity in the hepatitis phase suggests the possibility of early liver cancer