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目的:观察人参皂苷Rg1(Rg1)诱导人白血病K562细胞株衰老的作用及其机制。方法:MTT比色法检测Rg1对K562细胞增殖的影响,筛选最佳作用浓度及时间(20μmol.L-1,48 h)。流式细胞术检测Rg1对细胞周期的影响;SA-β-Gal染色检测细胞阳性染色百分率;RT-PCR法检测衰老相关基因p16,p53,p21,Rb的表达;电镜观察细胞衰老超微形态学改变。结果:Rg1在体外能明显抑制K562细胞增殖,使细胞阻滞于G2/M期;SA-β-Gal染色阳性细胞百分率显著增高(P<0.05);细胞衰老相关基因的表达上调(P<0.05);超微结构观察显示细胞增大,异染色质凝集、碎裂,线粒体体积增大,溶酶体体积增大、数目增多等衰老形态学变化。结论:Rg1能诱导K562细胞衰老,p53-p21-Rb,p16-Rb信号转导通路在其衰老调控中起重要作用。
Objective: To observe the effect of ginsenoside Rg1 (Rg1) on the senescence of human leukemia K562 cell line and its mechanism. Methods: MTT assay was used to detect the effect of Rg1 on the proliferation of K562 cells. The optimal concentration and time (20μmol.L-1,48 h) were screened. Flow cytometry was used to detect the effect of Rg1 on cell cycle. The percentage of positive cells was detected by SA-β-Gal staining. The expression of p16, p53, p21 and Rb were detected by RT-PCR. change. Results: Rg1 could inhibit the proliferation of K562 cells in vitro and arrest the cells in G2 / M phase. The percentage of SA-β-Gal positive cells was significantly increased (P <0.05), and the expression of senescence related genes was up-regulated ); Ultrastructural observation showed that the cells increased, heterochromatin aggregation, fragmentation, increased mitochondria volume, lysosome volume increased, the number of aging morphological changes. Conclusion: Rg1 can induce the senescence of K562 cells, and p53-p21-Rb and p16-Rb signal transduction pathways play an important role in the regulation of aging.